{"id":7502,"date":"2026-08-14T07:31:18","date_gmt":"2026-08-14T07:31:18","guid":{"rendered":"https:\/\/www.mediapreparators.com\/blog\/facteurs-affectant-la-sterilite-lors-de-la-preparation-des-milieux-de-culture\/"},"modified":"2026-08-14T08:20:25","modified_gmt":"2026-08-14T08:20:25","slug":"facteurs-affectant-la-sterilite-lors-de-la-preparation-des-milieux-de-culture","status":"publish","type":"blog","link":"https:\/\/www.mediapreparators.com\/fr\/blog\/facteurs-affectant-la-sterilite-lors-de-la-preparation-des-milieux-de-culture\/","title":{"rendered":"Facteurs affectant la st\u00e9rilit\u00e9 lors de la pr\u00e9paration des milieux de culture"},"content":{"rendered":"<span class=\"span-reading-time rt-reading-time\" style=\"display: block;\"><span class=\"rt-label rt-prefix\">Tiempo de lectura:<\/span> <span class=\"rt-time\"> 4<\/span> <span class=\"rt-label rt-postfix\">minutos<\/span><\/span>\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"7502\" class=\"elementor elementor-7502 elementor-7470\" data-elementor-post-type=\"blog\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e1f630e e-flex e-con-boxed e-con e-parent\" data-id=\"e1f630e\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-f20dd46 e-con-full e-flex e-con e-child\" data-id=\"f20dd46\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-009ee50 elementor-widget elementor-widget-text-editor\" data-id=\"009ee50\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><a href=\"https:\/\/www.mediapreparators.com\/fr\/blog\/\">Blog &gt;<\/a><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e3435ee elementor-widget elementor-widget-text-editor\" data-id=\"e3435ee\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tFacteurs affectant la st\u00e9rilit\u00e9 lors de la pr\u00e9paration des milieux de culture\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7ff2ecc e-con-full e-flex e-con e-child\" data-id=\"7ff2ecc\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-072a703 e-con-full e-flex e-con e-child\" data-id=\"072a703\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2a497ea elementor-widget elementor-widget-heading\" data-id=\"2a497ea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Facteurs affectant la st\u00e9rilit\u00e9 lors de la pr\u00e9paration des milieux de culture<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-eb6de92 elementor-widget elementor-widget-text-editor\" data-id=\"eb6de92\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tD\u00e9couvrez les principaux facteurs qui affectent la st\u00e9rilit\u00e9 lors de la pr\u00e9paration des milieux de culture et comment pr\u00e9venir les erreurs et les contaminations afin de garantir des r\u00e9sultats fiables.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7642c33 elementor-icon-list--layout-inline elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"7642c33\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"elementor-icon-list-items elementor-inline-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">RAYPA Lab<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\"><span class=\"span-reading-time rt-reading-time\"><span class=\"rt-label rt-prefix\"><\/span> <span class=\"rt-time\"> 4<\/span> <span class=\"rt-label rt-postfix\">min de lecture<\/span><\/span><\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item elementor-inline-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Mis \u00e0 jour : 14\/08\/2026<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c38b46b e-con-full e-flex e-con e-parent\" data-id=\"c38b46b\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6e491b2 elementor-widget elementor-widget-progress-tracker\" data-id=\"6e491b2\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;percentage&quot;:&quot;yes&quot;,&quot;percentage_color&quot;:&quot;#FFFFFF&quot;,&quot;sticky&quot;:&quot;top&quot;,&quot;sticky_offset&quot;:72,&quot;type&quot;:&quot;horizontal&quot;,&quot;relative_to&quot;:&quot;entire_page&quot;,&quot;sticky_on&quot;:[&quot;desktop&quot;,&quot;tablet&quot;,&quot;mobile&quot;],&quot;sticky_effects_offset&quot;:0,&quot;sticky_anchor_link_offset&quot;:0}\" data-widget_type=\"progress-tracker.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\n\t\t<div class=\"elementor-scrolling-tracker elementor-scrolling-tracker-horizontal elementor-scrolling-tracker-alignment-\">\n\t\t\t\t\t\t<div class=\"current-progress\">\n\t\t\t\t\t<div class=\"current-progress-percentage\"><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<article class=\"elementor-element elementor-element-a0492fe e-flex e-con-boxed e-con e-parent\" data-id=\"a0492fe\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-ac56ad1 e-con-full e-flex e-con e-child\" data-id=\"ac56ad1\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-d68209e e-con-full e-flex e-con e-child\" data-id=\"d68209e\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;sticky&quot;:&quot;top&quot;,&quot;sticky_offset&quot;:100,&quot;sticky_effects_offset&quot;:100,&quot;sticky_parent&quot;:&quot;yes&quot;,&quot;sticky_on&quot;:[&quot;desktop&quot;,&quot;tablet&quot;,&quot;mobile&quot;],&quot;sticky_anchor_link_offset&quot;:0}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-aea318f elementor-toc--minimized-on-mobile elementor-widget elementor-widget-table-of-contents\" data-id=\"aea318f\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;headings_by_tags&quot;:[&quot;h2&quot;,&quot;h3&quot;],&quot;exclude_headings_by_selector&quot;:[],&quot;marker_view&quot;:&quot;bullets&quot;,&quot;icon&quot;:{&quot;value&quot;:&quot;&quot;,&quot;library&quot;:&quot;&quot;},&quot;no_headings_message&quot;:&quot;No se encontraron t\\u00edtulos en esta p\\u00e1gina.&quot;,&quot;collapse_subitems&quot;:&quot;yes&quot;,&quot;minimized_on&quot;:&quot;mobile&quot;,&quot;minimize_box&quot;:&quot;yes&quot;,&quot;hierarchical_view&quot;:&quot;yes&quot;,&quot;min_height&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;min_height_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;min_height_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"table-of-contents.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-toc__header\">\n\t\t\t\t\t\t<h4 class=\"elementor-toc__header-title\">\n\t\t\t\tSOMMAIRE\t\t\t<\/h4>\n\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-toc__toggle-button elementor-toc__toggle-button--expand\" role=\"button\" tabindex=\"0\" aria-controls=\"elementor-toc__aea318f\" aria-expanded=\"true\" aria-label=\"Ouvrir la table des mati\u00e8res\"><svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-chevron-down\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M207.029 381.476L12.686 187.132c-9.373-9.373-9.373-24.569 0-33.941l22.667-22.667c9.357-9.357 24.522-9.375 33.901-.04L224 284.505l154.745-154.021c9.379-9.335 24.544-9.317 33.901.04l22.667 22.667c9.373 9.373 9.373 24.569 0 33.941L240.971 381.476c-9.373 9.372-24.569 9.372-33.942 0z\"><\/path><\/svg><\/div>\n\t\t\t\t<div class=\"elementor-toc__toggle-button elementor-toc__toggle-button--collapse\" role=\"button\" tabindex=\"0\" aria-controls=\"elementor-toc__aea318f\" aria-expanded=\"true\" aria-label=\"Fermer la table des mati\u00e8res\"><svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-chevron-up\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M240.971 130.524l194.343 194.343c9.373 9.373 9.373 24.569 0 33.941l-22.667 22.667c-9.357 9.357-24.522 9.375-33.901.04L224 227.495 69.255 381.516c-9.379 9.335-24.544 9.317-33.901-.04l-22.667-22.667c-9.373-9.373-9.373-24.569 0-33.941L207.03 130.525c9.372-9.373 24.568-9.373 33.941-.001z\"><\/path><\/svg><\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<div id=\"elementor-toc__aea318f\" class=\"elementor-toc__body elementor-toc__list-items--collapsible\">\n\t\t\t<div class=\"elementor-toc__spinner-container\">\n\t\t\t\t<svg class=\"elementor-toc__spinner eicon-animation-spin e-font-icon-svg e-eicon-loading\" aria-hidden=\"true\" viewBox=\"0 0 1000 1000\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M500 975V858C696 858 858 696 858 500S696 142 500 142 142 304 142 500H25C25 237 238 25 500 25S975 237 975 500 763 975 500 975Z\"><\/path><\/svg>\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b560b2a e-con-full e-flex e-con e-child\" data-id=\"b560b2a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-faa9b4e e-con-full e-flex e-con e-child\" data-id=\"faa9b4e\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f9f3bd8 elementor-widget elementor-widget-heading\" data-id=\"f9f3bd8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">R\u00e9sum\u00e9<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-920f20e parrafo elementor-widget elementor-widget-text-editor\" data-id=\"920f20e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>La st\u00e9rilit\u00e9 des milieux de culture d\u00e9pend d\u2019une formulation correcte, d\u2019une st\u00e9rilisation efficace et d\u2019une manipulation aseptique apr\u00e8s st\u00e9rilisation. Le contr\u00f4le du pH et des ingr\u00e9dients, l\u2019application d\u2019un cycle d\u2019autoclavage adapt\u00e9 ainsi qu\u2019une distribution et une conservation appropri\u00e9es sont essentiels pour pr\u00e9venir les contaminations et obtenir des r\u00e9sultats fiables. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-dca7399 e-con-full e-flex e-con e-child\" data-id=\"dca7399\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-b3208e0 e-con-full e-flex e-con e-child\" data-id=\"b3208e0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c24519d elementor-widget elementor-widget-heading\" data-id=\"c24519d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Pourquoi la st\u00e9rilit\u00e9 est-elle importante lors de la pr\u00e9paration des milieux de culture ?<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6e6ff26 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"6e6ff26\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>La st\u00e9rilit\u00e9 est une exigence essentielle pour \u00e9viter toute contamination susceptible de compromettre les exp\u00e9riences ou les processus de production. Cependant, maintenir la st\u00e9rilit\u00e9 n\u2019est pas toujours simple, car diff\u00e9rents facteurs peuvent l\u2019affecter tout au long du processus. <\/p><p>Voici les principaux \u00e9l\u00e9ments susceptibles d\u2019influencer la st\u00e9rilit\u00e9 lors de la pr\u00e9paration des milieux de culture, ainsi que les mesures \u00e0 mettre en \u0153uvre pour les ma\u00eetriser efficacement.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c66a923 e-con-full e-flex e-con e-child\" data-id=\"c66a923\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cdb373a elementor-widget elementor-widget-heading\" data-id=\"cdb373a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">1. Qualit\u00e9 de l\u2019eau utilis\u00e9e<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e2c4a58 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"e2c4a58\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : L\u2019eau \u00e9tant le principal composant des milieux de culture, sa puret\u00e9 est essentielle. L\u2019utilisation d\u2019une eau non trait\u00e9e ou de mauvaise qualit\u00e9 peut introduire des micro-organismes, des particules ou m\u00eame des ions m\u00e9talliques susceptibles de favoriser la croissance microbienne. <\/p><p><strong>Solution<\/strong> : Utiliser d\u00e8s le d\u00e9but du processus de l\u2019eau distill\u00e9e, d\u00e9ionis\u00e9e ou ultrapure afin de garantir l\u2019absence de contaminants. Cette \u00e9tape n\u2019a pas d\u2019incidence directe sur la st\u00e9rilit\u00e9, mais permet de pr\u00e9server la composition chimique du milieu. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-4b11ca6 e-con-full e-flex e-con e-child\" data-id=\"4b11ca6\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-976c788 e-con-full e-flex e-con e-child\" data-id=\"976c788\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-99421ce elementor-widget elementor-widget-heading\" data-id=\"99421ce\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">2. Contamination chimique et erreurs de formulation<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c1cc12f parrafo elementor-widget elementor-widget-text-editor\" data-id=\"c1cc12f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : La pr\u00e9sence de r\u00e9sidus chimiques, tels que des d\u00e9tergents, des d\u00e9sinfectants ou des huiles, peut alt\u00e9rer la composition du milieu et ces r\u00e9sidus ne sont pas \u00e9limin\u00e9s par la st\u00e9rilisation en autoclave. De m\u00eame, des erreurs lors de la pes\u00e9e des composants ou un ajustement incorrect du pH peuvent affecter les propri\u00e9t\u00e9s et l\u2019efficacit\u00e9 du milieu. <\/p><p><strong>Solution<\/strong> : Utiliser des r\u00e9cipients et des ustensiles correctement rinc\u00e9s et exempts de r\u00e9sidus chimiques, et v\u00e9rifier soigneusement la pes\u00e9e des composants ainsi que le pH avant la st\u00e9rilisation.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-96ea7e5 e-con-full e-flex e-con e-child\" data-id=\"96ea7e5\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d4d90f9 elementor-widget elementor-widget-heading\" data-id=\"d4d90f9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">3. St\u00e9rilisation insuffisante<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d6318a7 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"d6318a7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : Une st\u00e9rilisation inad\u00e9quate constitue l\u2019une des causes les plus fr\u00e9quentes de contamination. Si le cycle de l\u2019autoclave n\u2019atteint pas la temp\u00e9rature ou la dur\u00e9e requises, ou si l\u2019\u00e9quipement est mal \u00e9talonn\u00e9, certains micro-organismes peuvent survivre et contaminer le milieu. <\/p><p><strong>Solution<\/strong> : V\u00e9rifier le bon fonctionnement de l\u2019autoclave et s\u2019assurer que le milieu atteint au moins 121 \u00b0C pendant 15 \u00e0 20 minutes, en fonction du volume et des ingr\u00e9dients du milieu.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a73fb3c e-con-full e-flex e-con e-child\" data-id=\"a73fb3c\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-137090a elementor-widget elementor-widget-heading\" data-id=\"137090a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">4. Ajout de composants thermolabiles<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cfc0b17 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"cfc0b17\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : Certains composants des milieux de culture, tels que les antibiotiques, le sang, les vitamines ou les suppl\u00e9ments s\u00e9lectifs, sont thermolabiles et se d\u00e9gradent lors de la st\u00e9rilisation en autoclave. S\u2019ils sont ajout\u00e9s avant le cycle, ils peuvent perdre tout ou partie de leur activit\u00e9. Ils doivent donc \u00eatre ajout\u00e9s apr\u00e8s la st\u00e9rilisation, ce qui constitue une \u00e9tape critique en mati\u00e8re de risque de contamination.  <\/p><p><strong>Solution<\/strong> : Lorsque le milieu a atteint la temp\u00e9rature recommand\u00e9e pour chaque suppl\u00e9ment, ajouter les composants thermolabiles pr\u00e9alablement filtr\u00e9s en appliquant une technique aseptique afin de pr\u00e9server la st\u00e9rilit\u00e9 du milieu avant sa distribution. Il est important de maintenir les zones de travail propres, avec un contr\u00f4le de l\u2019air et un acc\u00e8s restreint. L\u2019utilisation de hottes \u00e0 flux laminaire ou de pr\u00e9parateurs de milieux \u00e9quip\u00e9s de ports d\u2019ajout sp\u00e9cifiques constitue une pratique essentielle pour \u00e9viter toute contamination environnementale pendant la pr\u00e9paration.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-74cc654 e-con-full e-flex e-con e-child\" data-id=\"74cc654\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c18bdf5 elementor-widget elementor-widget-heading\" data-id=\"c18bdf5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">5. Environnement de travail<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-acda822 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"acda822\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : L\u2019environnement dans lequel le milieu est distribu\u00e9 joue un r\u00f4le d\u00e9terminant dans le maintien de la st\u00e9rilit\u00e9. La pr\u00e9sence de poussi\u00e8res, de spores fongiques ou de bact\u00e9ries en suspension augmente le risque de contamination. <\/p><p><strong>Solution<\/strong> : Maintenir les zones de travail propres, avec un contr\u00f4le de l\u2019air et un acc\u00e8s restreint. L\u2019utilisation de hottes \u00e0 flux laminaire constitue une pratique essentielle pour emp\u00eacher les contaminants environnementaux d\u2019atteindre le milieu lors de sa distribution. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d5e1c96 e-con-full e-flex e-con e-child\" data-id=\"d5e1c96\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-065cc17 elementor-widget elementor-widget-heading\" data-id=\"065cc17\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">6. \u00c9quipements et r\u00e9cipients mal st\u00e9rilis\u00e9s<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-816f4a2 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"816f4a2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : Les r\u00e9cipients r\u00e9utilisables, les agitateurs et les ustensiles peuvent constituer des sources de contamination s\u2019ils ne sont pas correctement st\u00e9rilis\u00e9s. Cela concerne notamment les flacons, les spatules et tout autre outil entrant en contact avec le milieu. <\/p><p><strong>Solution<\/strong> : St\u00e9riliser tous les \u00e9quipements entrant en contact avec le milieu, par autoclavage ou au moyen de techniques de d\u00e9sinfection chimique, afin de garantir un environnement exempt de contaminants.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-27dc6d0 e-con-full e-flex e-con e-child\" data-id=\"27dc6d0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-706bd70 elementor-widget elementor-widget-heading\" data-id=\"706bd70\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">7. Dur\u00e9e et temp\u00e9rature de refroidissement apr\u00e8s st\u00e9rilisation<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-621373d parrafo elementor-widget elementor-widget-text-editor\" data-id=\"621373d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : Apr\u00e8s la st\u00e9rilisation, un refroidissement trop lent ou une exposition prolong\u00e9e \u00e0 l\u2019environnement peut favoriser l\u2019introduction de micro-organismes.<\/p><p><strong>Solution<\/strong> : Refroidir le milieu dans des zones st\u00e9riles et le fermer herm\u00e9tiquement d\u00e8s qu\u2019il atteint la temp\u00e9rature appropri\u00e9e pour sa manipulation ou son stockage.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2401801 e-con-full e-flex e-con e-child\" data-id=\"2401801\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a73efec elementor-widget elementor-widget-heading\" data-id=\"a73efec\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">8. Fermeture et stockage<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c88dbb3 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"c88dbb3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Probl\u00e8me<\/strong> : Des conditions de stockage inad\u00e9quates apr\u00e8s la st\u00e9rilisation peuvent compromettre la qualit\u00e9 et la st\u00e9rilit\u00e9 du milieu de culture.<\/p><p><strong>Solution<\/strong> : Maintenir les milieux correctement ferm\u00e9s et les conserver au r\u00e9frig\u00e9rateur, g\u00e9n\u00e9ralement entre 2 et 8 \u00b0C. Dans le cas de milieux solides distribu\u00e9s dans des bo\u00eetes de Petri, les conserver au r\u00e9frig\u00e9rateur et en position retourn\u00e9e afin d\u2019\u00e9viter que la condensation ne s\u2019accumule \u00e0 la surface du milieu. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-4a4a9e8 e-con-full e-flex e-con e-child\" data-id=\"4a4a9e8\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-46af232 elementor-widget elementor-widget-heading\" data-id=\"46af232\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Comment garantir la st\u00e9rilit\u00e9 tout au long du processus<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df8c520 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"df8c520\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Pour pr\u00e9server la st\u00e9rilit\u00e9 \u00e0 chaque \u00e9tape, il est essentiel de mettre en \u0153uvre des pratiques de contr\u00f4le rigoureuses, notamment :<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f331504 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"f331504\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>Utiliser de l\u2019eau ultrapure d\u00e8s le d\u00e9but du processus.<\/li><li>Effectuer la pr\u00e9paration sous hotte \u00e0 flux laminaire ou dans des zones de travail st\u00e9riles.<\/li><li>St\u00e9riliser correctement les \u00e9quipements et les r\u00e9cipients \u00e0 l\u2019aide d\u2019autoclaves correctement \u00e9tal\u00e9s.<\/li><li>Manipuler les ingr\u00e9dients et le milieu dans des conditions d\u2019asepsie rigoureuses.<\/li><li>Fermer herm\u00e9tiquement les milieux et les conserver dans des conditions contr\u00f4l\u00e9es.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1b0b2d8 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"1b0b2d8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>La st\u00e9rilit\u00e9 n\u2019est pas seulement un crit\u00e8re de qualit\u00e9 : elle constitue une exigence indispensable pour garantir des r\u00e9sultats fiables et reproductibles. Investir dans des \u00e9quipements modernes, tels que des pr\u00e9parateurs de milieux dot\u00e9s de syst\u00e8mes automatis\u00e9s de st\u00e9rilisation, peut consid\u00e9rablement faciliter ce processus, en r\u00e9duisant les risques et en optimisant les temps de pr\u00e9paration. <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-6ce2b5f e-con-full e-flex e-con e-child\" data-id=\"6ce2b5f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t<div class=\"elementor-element elementor-element-af827f1 e-con-full e-flex e-con e-child\" data-id=\"af827f1\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-044ea53 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"044ea53\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Vous souhaitez en savoir plus sur les moyens d\u2019am\u00e9liorer la pr\u00e9paration des milieux dans votre laboratoire ?<\/strong><\/p><p>Contactez notre \u00e9quipe : nous vous aiderons \u00e0 trouver la solution la mieux adapt\u00e9e \u00e0 votre application.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b63b00d elementor-align-center elementor-widget elementor-widget-button\" data-id=\"b63b00d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-sm\" href=\"https:\/\/portal.raypa.com\/contact?marca=mp&#038;lang=fr\" target=\"_blank\" rel=\"noopener\">\n\t\t\t\t\t\t<span 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class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\u00c9quipe de professionnels sp\u00e9cialis\u00e9e dans la st\u00e9rilisation par autoclave et la pr\u00e9paration de milieux de culture, avec une expertise en microbiologie, contr\u00f4le qualit\u00e9 et biotechnologie.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2f649a2 e-con-full e-flex e-con e-child\" data-id=\"2f649a2\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e5dc642 parrafo elementor-widget elementor-widget-text-editor\" data-id=\"e5dc642\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Partager<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-67ae5ae elementor-share-buttons--shape-circle elementor-share-buttons--color-custom elementor-share-buttons--view-icon-text elementor-share-buttons--skin-gradient elementor-grid-0 elementor-widget elementor-widget-share-buttons\" data-id=\"67ae5ae\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"share-buttons.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-grid\" role=\"list\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-grid-item\" role=\"listitem\">\n\t\t\t\t\t\t<div class=\"elementor-share-btn elementor-share-btn_whatsapp\" role=\"button\" tabindex=\"0\" aria-label=\"Partager sur whatsapp\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-share-btn__icon\">\n\t\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fab-whatsapp\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M380.9 97.1C339 55.1 283.2 32 223.9 32c-122.4 0-222 99.6-222 222 0 39.1 10.2 77.3 29.6 111L0 480l117.7-30.9c32.4 17.7 68.9 27 106.1 27h.1c122.3 0 224.1-99.6 224.1-222 0-59.3-25.2-115-67.1-157zm-157 341.6c-33.2 0-65.7-8.9-94-25.7l-6.7-4-69.8 18.3L72 359.2l-4.4-7c-18.5-29.4-28.2-63.3-28.2-98.2 0-101.7 82.8-184.5 184.6-184.5 49.3 0 95.6 19.2 130.4 54.1 34.8 34.9 56.2 81.2 56.1 130.5 0 101.8-84.9 184.6-186.6 184.6zm101.2-138.2c-5.5-2.8-32.8-16.2-37.9-18-5.1-1.9-8.8-2.8-12.5 2.8-3.7 5.6-14.3 18-17.6 21.8-3.2 3.7-6.5 4.2-12 1.4-32.6-16.3-54-29.1-75.5-66-5.7-9.8 5.7-9.1 16.3-30.3 1.8-3.7.9-6.9-.5-9.7-1.4-2.8-12.5-30.1-17.1-41.2-4.5-10.8-9.1-9.3-12.5-9.5-3.2-.2-6.9-.2-10.6-.2-3.7 0-9.7 1.4-14.8 6.9-5.1 5.6-19.4 19-19.4 46.3 0 27.3 19.9 53.7 22.6 57.4 2.8 3.7 39.1 59.7 94.8 83.8 35.2 15.2 49 16.5 66.6 13.9 10.7-1.6 32.8-13.4 37.4-26.4 4.6-13 4.6-24.1 3.2-26.4-1.3-2.5-5-3.9-10.5-6.6z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-grid-item\" role=\"listitem\">\n\t\t\t\t\t\t<div class=\"elementor-share-btn elementor-share-btn_email\" role=\"button\" tabindex=\"0\" aria-label=\"Partager sur email\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-share-btn__icon\">\n\t\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fas-envelope\" viewBox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M502.3 190.8c3.9-3.1 9.7-.2 9.7 4.7V400c0 26.5-21.5 48-48 48H48c-26.5 0-48-21.5-48-48V195.6c0-5 5.7-7.8 9.7-4.7 22.4 17.4 52.1 39.5 154.1 113.6 21.1 15.4 56.7 47.8 92.2 47.6 35.7.3 72-32.8 92.3-47.6 102-74.1 131.6-96.3 154-113.7zM256 320c23.2.4 56.6-29.2 73.4-41.4 132.7-96.3 142.8-104.7 173.4-128.7 5.8-4.5 9.2-11.5 9.2-18.9v-19c0-26.5-21.5-48-48-48H48C21.5 64 0 85.5 0 112v19c0 7.4 3.4 14.3 9.2 18.9 30.6 23.9 40.7 32.4 173.4 128.7 16.8 12.2 50.2 41.8 73.4 41.4z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-grid-item\" role=\"listitem\">\n\t\t\t\t\t\t<div class=\"elementor-share-btn elementor-share-btn_linkedin\" role=\"button\" tabindex=\"0\" aria-label=\"Partager sur linkedin\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-share-btn__icon\">\n\t\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fab-linkedin\" viewBox=\"0 0 448 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M416 32H31.9C14.3 32 0 46.5 0 64.3v383.4C0 465.5 14.3 480 31.9 480H416c17.6 0 32-14.5 32-32.3V64.3c0-17.8-14.4-32.3-32-32.3zM135.4 416H69V202.2h66.5V416zm-33.2-243c-21.3 0-38.5-17.3-38.5-38.5S80.9 96 102.2 96c21.2 0 38.5 17.3 38.5 38.5 0 21.3-17.2 38.5-38.5 38.5zm282.1 243h-66.4V312c0-24.8-.5-56.7-34.5-56.7-34.6 0-39.9 27-39.9 54.9V416h-66.4V202.2h63.7v29.2h.9c8.9-16.8 30.6-34.5 62.9-34.5 67.2 0 79.7 44.3 79.7 101.9V416z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-grid-item\" role=\"listitem\">\n\t\t\t\t\t\t<div class=\"elementor-share-btn elementor-share-btn_facebook\" role=\"button\" tabindex=\"0\" aria-label=\"Partager sur facebook\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-share-btn__icon\">\n\t\t\t\t\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-fab-facebook\" viewBox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M504 256C504 119 393 8 256 8S8 119 8 256c0 123.78 90.69 226.38 209.25 245V327.69h-63V256h63v-54.64c0-62.15 37-96.48 93.67-96.48 27.14 0 55.52 4.84 55.52 4.84v61h-31.28c-30.8 0-40.41 19.12-40.41 38.73V256h68.78l-11 71.69h-57.78V501C413.31 482.38 504 379.78 504 256z\"><\/path><\/svg>\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-5253306 e-con-full elementor-hidden-desktop elementor-hidden-tablet elementor-hidden-mobile e-flex e-con e-child\" data-id=\"5253306\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-574778a elementor-widget elementor-widget-heading\" data-id=\"574778a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">Fais \u00e9voluer votre expertise<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b05329b parrafo elementor-widget elementor-widget-text-editor\" data-id=\"b05329b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Abonnez-vous pour recevoir des contenus sp\u00e9cialis\u00e9s sur les milieux de culture, la st\u00e9rilisation, l\u2019automatisation et les biotechnologies v\u00e9g\u00e9tales.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7ef8b4b elementor-button-align-stretch elementor-widget elementor-widget-form\" data-id=\"7ef8b4b\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;step_next_label&quot;:&quot;Next&quot;,&quot;step_previous_label&quot;:&quot;Previous&quot;,&quot;step_type&quot;:&quot;none&quot;,&quot;button_width&quot;:&quot;100&quot;,&quot;step_icon_shape&quot;:&quot;circle&quot;}\" data-widget_type=\"form.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<form class=\"elementor-form\" method=\"post\" name=\"Newsletter\" aria-label=\"Newsletter\">\n\t\t\t<input type=\"hidden\" name=\"post_id\" value=\"7502\"\/>\n\t\t\t<input type=\"hidden\" name=\"form_id\" value=\"7ef8b4b\"\/>\n\t\t\t<input type=\"hidden\" name=\"referer_title\" value=\"\" \/>\n\n\t\t\t\n\t\t\t<div class=\"elementor-form-fields-wrapper elementor-labels-\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-field-type-email elementor-field-group elementor-column elementor-field-group-email elementor-col-100 elementor-field-required\">\n\t\t\t\t\t\t\t\t\t\t\t\t<label for=\"form-field-email\" class=\"elementor-field-label elementor-screen-only\">\n\t\t\t\t\t\t\t\tEmail\t\t\t\t\t\t\t<\/label>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<input size=\"1\" type=\"email\" name=\"form_fields[email]\" id=\"form-field-email\" class=\"elementor-field elementor-size-sm  elementor-field-textual\" placeholder=\"Email\" required=\"required\">\n\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<div class=\"elementor-field-group elementor-column elementor-field-type-submit elementor-col-100 e-form__buttons\">\n\t\t\t\t\t<button class=\"elementor-button elementor-size-sm\" type=\"submit\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">S\u2019abonner<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/button>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t<\/form>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/article>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p><span class=\"span-reading-time rt-reading-time\" style=\"display: block;\"><span class=\"rt-label rt-prefix\">Tiempo de lectura:<\/span> <span class=\"rt-time\"> 4<\/span> <span class=\"rt-label rt-postfix\">minutos<\/span><\/span>D\u00e9couvrez les principaux facteurs qui affectent la st\u00e9rilit\u00e9 lors de la pr\u00e9paration des milieux de culture et comment pr\u00e9venir les erreurs et les contaminations afin de garantir des r\u00e9sultats fiables.<\/p>\n","protected":false},"author":5,"featured_media":7494,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categoria-de-articulo":[41],"class_list":["post-7502","blog","type-blog","status-publish","format-standard","has-post-thumbnail","hentry","categoria-de-articulo-milieux-de-culture"],"acf":{"imagen_categoria_articulo":null},"_links":{"self":[{"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/blog\/7502","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/blog"}],"about":[{"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/types\/blog"}],"author":[{"embeddable":true,"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/comments?post=7502"}],"version-history":[{"count":1,"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/blog\/7502\/revisions"}],"predecessor-version":[{"id":7503,"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/blog\/7502\/revisions\/7503"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/media\/7494"}],"wp:attachment":[{"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/media?parent=7502"}],"wp:term":[{"taxonomy":"categoria-de-articulo","embeddable":true,"href":"https:\/\/www.mediapreparators.com\/fr\/wp-json\/wp\/v2\/categoria-de-articulo?post=7502"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}